Table 2

Comparison of the activation and deactivation kinetics of TRPV1. Mean activation and deactivation rates of responses from TRPV1 expressing HEK-293 cells following application of capsaicin (1 μM), acidic pH (5.5) or the combination. Row 3 represents activation and deactivation rates of currents evoked by 1 μM capsaicin following pre-exposure to acidic conditions (pH 5.5). Row 4 represents the activation rates of acid-evoked currents following pre-exposure to capsaicin (1 μM). The absolute value of rate measurements had slight day-to-day variations (possibly due to relative position of the drug application tube). Therefore values in this table are from cells in which direct comparisons can be made. Values are mean ± S.E.M.

Activation Rates

Deactivation Rates


τ (ms)

n

τ (ms)

n


1 μM Capsaicin

847 ± 119

4

845 ± 60

4

pH 5.5

917 ± 307

3

56 ± 13

4

1 μM CAP (pH 5.5)

149.3 ± 17

4

10553 ± 1880

4

pH 5.5 (1 μM CAP)

93 ± 13

3

-not tested-


Note: Rates from experiments shown in Figures 4 and 5.

Neelands et al. Molecular Pain 2005 1:28   doi:10.1186/1744-8069-1-28

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