Table 1

Antisera.

Antisera

Species

Dilution

Source


ASIC3

guinea pig

rabbit

1:300

1:1000

Neuromics

Robert Elde, University of Minnesota, Minneapolis

P2X3

rabbit

1:2000

Neuromics

TRPV1

rabbit

guinea pig

1:1000

1:1000

Neuromics

Neuromics

TrkA

rabbit

1:300

Chemicon, Temecula, CA

TrkC

Goat

1:300

Louis Reichardt, Universityof California, SF

Parvalbumin

mouse

1:1000

Sigma, St. Louis, MO

N52

mouse

1:10000

Sigma

CGRP

mouse

rabbit

guinea pig

1:2000

1:1000

1:500

RBI, Natick, MA

Chemicon

Peninsula

NPY

Sheep

1:1000

Chemicon

NF200

rabbit

1:1000

Chemicon


The guinea pig anti-ASIC3 was used to label all DRG (Figs. 1-6). Both guinea pig and rabbit anti-ASIC3 were used to label vascular innervation; when used together, each blocked labelling by the other. Fig. 7 shows results with the rabbit anti-ASIC3.

Molliver et al. Molecular Pain 2005 1:35   doi:10.1186/1744-8069-1-35

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