Impact of paclitaxel and long term drug infusions on mRNA levels of astrocytes, microglia, and cannabinoid receptors in lumbar spinal cord. a. Paclitaxel marginally altered long term expression of mRNA markers of astrocytes (GFAP) but not microglia (CD11b) in lumbar spinal cord (L4-L6) tissue samples relative to cremophor conditions. Chronic infusions of the mixed CB1/CB2 agonist WIN55,212-2 (0.5 mg/kg/day s.c.) and the CB2-preferring agonist AM1710 (3.2 mg/kg/day s.c.) did not alter either GFAP or CD11b b. but increased both CB1 and CB2 mRNA expression in the same tissues. Transcriptional regulation of CB1 and CB2 mRNAs were blocked by the CB2 antagonist AM630 (3 mg/kg/day s.c.). +P = 0.059 vs. cremophor-vehicle (planned comparison one-tailed t-test), **P < 0.01, ***P < 0.001, vs. all groups except Taxol-WIN55,212-2 (0.5 mg/kg/day s.c.), # P < 0.05 vs. Taxol-Vehicle, Cremophor-Vehicle, and Taxol-WIN55,212-2 (0.5) + AM630 (3) (ANOVA; Dunnett and Tukey post hoc tests). N = 4 per group.
Rahn et al. Molecular Pain 2014 10:27 doi:10.1186/1744-8069-10-27