Inhibition of NF-κB prevents the up-regulation of mGlu2 mRNA induced by L-acetylcarnitine treatment. CAPE (2.5 μg/ml) was applied to DRG cultures 30 minutes before L-acetylcarnitine (LAC) (250 μM). 1 hr after LAC treatment total RNA was extracted. mRNA levels were normalized to the housekeeping gene, GAPDH, and compared to controls. Real-time PCR analysis shows that mGlu2 mRNA upregulation induced by LAC is blocked by CAPE treatment. Data are expressed as mean values ± SEM *p < 0.05 vs controls, #p < 0.05 vs LAC (ANOVA).
Chiechio et al. Molecular Pain 2006 2:20 doi:10.1186/1744-8069-2-20