Figure 1.

Long-lasting and bilateral allodynia and hyperalgesia induced by ICS. A, B: ICS-induced mechanical allodynia on the (A) right and (B) left hind paws. The paw pressure test, using digital von Frey apparatus, was performed at pre-stress (day 0) and at P1, 5 and 12 (days 3, 7 and 14) after ICS/CCS. C: Effects of gonadectomy on ICS-induced mechanical allodynia in male and female mice. Data represent the decrease in the threshold obtained by subtracting the threshold at P1 or P5 from the pre-stress value. D: ICS-induced thermal hyperalgesia. Data represent the means ± S.E.M. from 4–6 individual mice per group. *p < 0.05 versus control group. #p < 0.05 versus sham group.