Altered TRPV1 staining is observed in DRG obtained from STZ-induced diabetic mice. A. Representative TRPV1 immunofluorescent pictures of DRG sections from control non-diabetic (a), STZ-induced diabetic hyperalgesic (b), hypoalgesic mice (c) and non-diabetic TRPV1 knock-out mice (d). B. Summary graph showing increase in mean gray values of TRPV1 staining (n = 5 sections from 4 mice, p < 0.058) in hyperalgesic DRG and decrease in TRPV1 gray value in hypoalgesic diabetic mice (n = 6 sections from 4 mice, p < 0.05) as compared to control non-diabetic mice. C. Percentage of TRPV1-expressing neurons in total DRG neuron was higher (n = 5 sections from 4 mice, p < 0.05) in hyperalgesia and did not change in hypoalgesic (n = 6 sections) diabetic mice as compared to age matched control non-diabetic mice. Note that TRPV1knock-out mice sections did not exhibit any TRPV1 staining serving as negative control. Number in the parenthesis represents the number of TRPV1 stained sections and the asterisk (*) represents p < 0.05. Scale bar is 50 μm.
Pabbidi et al. Molecular Pain 2008 4:9 doi:10.1186/1744-8069-4-9