Figure 3.

Blockade of JWH015's effects on MKP-1 and MKP-3. (A) Representative western blots of MKP-1 and beta-actin in primary microglia treated with LPS in the absence or presence of JWH015 (15 min incubation, JWH). Cells were pre-incubated with or without 10 μM PSI2106 (PS), Ro-31-8220 (Ro) or triptolide (Trip) for 30 min. (B) Triptolide (10 μM) completely blocked the MKP-1 expression induced by JWH015 (1 μM). (C) PSI2106 or Ro-31-8220 partially inhibited the MKP-1 expression induced by JWH015. (D) Representative western blots of MKP-3 and beta-actin in primary microglia treated with LPS in the absence of presence of JWH015 (15 min incubation). Cells were pre-incubated with or without PSI2106, Ro-31-8220 or triptolide (10 μM) for 30 min. (E) Triptolide (10 μM) completely blocked the MKP-3 expression induced by JWH015 (1 μM). (F) PSI2106 or Ro-31-8220 did not modify the MKP-3 expression induced by JWH015. *p < 0.05 vs. LPS + medium (MED) or LPS + JWH015 + PSI2106, Ro-31-8220 or triptolide group (indicated by the connecting lines).