Research

Morphine modulation of pain processing in medial and lateral pain pathways

Jin-Yan Wang^1* , Jin Huang^2* , Jing-Yu Chang³ , Donald J Woodward³ and Fei Luo¹

¹  Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Science, Beijing, China

²  Neuroscience Research Institute, Peking University, Beijing, China

³  Neuroscience Research Institute of North Carolina, Winston-Salem, NC, USA

author email corresponding author email* Contributed equally

Molecular Pain 2009, 5:60doi:10.1186/1744-8069-5-60

Published:	13 October 2009

Abstract

Background

Despite the wide-spread use of morphine and related opioid agonists in clinic and their powerful analgesic effects, our understanding of the neural mechanisms underlying opioid analgesia at supraspinal levels is quite limited. The present study was designed to investigate the modulative effect of morphine on nociceptive processing in the medial and lateral pain pathways using a multiple single-unit recording technique. Pain evoked neuronal activities were simultaneously recorded from the primary somatosensory cortex (SI), ventral posterolateral thalamus (VPL), anterior cingulate cortex (ACC), and medial dorsal thalamus (MD) with eight-wire microelectrode arrays in awake rats.

Results

The results showed that the noxious heat evoked responses of single neurons in all of the four areas were depressed after systemic injection of 5 mg/kg morphine. The depressive effects of morphine included (i) decreasing the neuronal response magnitude; (ii) reducing the fraction of responding neurons, and (iii) shortening the response duration. In addition, the capability of cortical and thalamic neural ensembles to discriminate noxious from innocuous stimuli was decreased by morphine within both pain pathways. Meanwhile, morphine suppressed the pain-evoked changes in the information flow from medial to lateral pathway and from cortex to thalamus. These effects were completely blocked by pre-treatment with the opiate receptor antagonist naloxone.

Conclusion

These results suggest that morphine exerts analgesic effects through suppressing both sensory and affective dimensions of pain.