Lysophosphatidic acid-3 receptor-mediated feed-forward production of lysophosphatidic acid: an initiator of nerve injury-induced neuropathic pain

doi:10.1186/1744-8069-5-64

Molecular Pain

Volume 5

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Aoki J
Ueda H

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Lysophosphatidic acid-3 receptor-mediated feed-forward production of lysophosphatidic acid: an initiator of nerve injury-induced neuropathic pain

Lin Ma¹ , Hitoshi Uchida¹ , Jun Nagai¹ , Makoto Inoue¹ , Jerold Chun² , Junken Aoki³ and Hiroshi Ueda¹

¹Division of Molecular Pharmacology and Neuroscience, Nagasaki University Graduate School of Biomedical Sciences, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan

²Department of Molecular Biology, Helen L. Dorris Child and Adolescent Neuropsychiatric Disorder Institute, The Scripps Research Institute, 10550 North Torrey Pines Road, ICND118, La Jolla, CA 92037, USA

³Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan

author email corresponding author email

Molecular Pain 2009, 5:64doi:10.1186/1744-8069-5-64


Published:	13 November 2009

Additional files

Additional file 1:

Specificity of LPA measurements using a measure of cell rounding activity in B103 cells. (a) Cell rounding activity of added LPA or S1P to B103 (+) cells. (b) Cell rounding activity of added LPA or tissue extracts to B103 (-) cells. (c) Linearity (y = 5.454x + 5.66, R²= 0.991) of cell rounding-inducing activity for LPA between 0.15 and 5 pmol in B103 (+) cells, after subtracting the basal activity. All data represent the mean ± SEM from three to four separate experiments. *p < 0.05 compared with the vehicle group. Other details are shown in the legend of Figure 1.

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Additional file 2:

Gene expression analysis for LPA₁, LPA₂and LPA₃receptors in the spinal cord dorsal horn and dorsal root. Expression of LPA₁, LPA₂and LPA₃receptors in mouse spinal cord dorsal horn (SC) and dorsal root (DR) by RT-PCR.

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