Figure 1.

L5 spinal nerve ligation (SNL) alters the expression of mRNAs in the ipsilateral L5DRG. A, Dark-field micrographs display hybridization signals for each of the six transcripts studied: Scn10a, Scn11a, P2rx3, Trpv1, Trpa1 and Trpm8. DRG sections originate from naïve B6 mice (left column) and B6 mice 3 days after SNL surgery (right column). The scale bar refers to all images: 100 μm. B, Column heights (mean ± SEM, n = 4–6) indicate the proportion of DRG neuronal profiles that were positive for expression of each of the six mRNA types in each of the five strains examined. White columns refer to naïve mice; black columns refer to SNL operated mice. Statistical differences between naive and nerve injured mice are indicated with asterisks (Mann-Whitney U test) * p < 0.05; ** p < 0.01.