Agarose gel electrophoresis (upper panel, two experiments) and quantitative comparison (lower panel) of RT-PCR-amplified products of the IL-1β mRNAs from rat Vi/Vc tissues. A. Effect of IL-10. B. Effect of fluorocitrate (FC) and minocycline (MC). Compared to naive controls (N), there were significant increases in IL-1β mRNAs in the Vi/Vc transition zone after CFA-induced masseter inflammation, which was reversed in rats receiving IL-10, FC or MC. Asterisks denote significant differences from CFA or Sal-CFA, **, P < 0.01. Error bars represent S.E.M.
Shimizu et al. Molecular Pain 2009 5:75 doi:10.1186/1744-8069-5-75