Caffeine-induced LTP of fEPSPs in spinal cord slice. A, After perfusion with 10 mM caffeine for 2 min instantly following washed out, LTP of fEPSPs occurred without conditioning stimulation in the presence of 10 μM bicuculline methiodide and 1 μM strychnine, which lasted for more than 80 min. Inserts show original recordings 5 min before (1) and about 80 min after (2) 10 mM caffeine challenge. Traces were not averaged (n = 13). B, Perfusion with 20 μM ryanodine 1 hour before caffeine challenge blocked the induction of LTP of fEPSPs. Inserts show original recordings without averaging (n = 5).
Cheng et al. Molecular Pain 2010 6:1 doi:10.1186/1744-8069-6-1