Table 2

PAR activators and concentrations used

PAR activator

Concentration

Selective for

Source

Thrombin

0.01-100 nM

PAR1,3,4

Sigma, cat T4648

Cathepsin G

1-1000 nM

PAR4

Sigma, cat C4428

Trypsin

0.1-10 μM

PAR1,2,3,4

Sigma, cat T9201

Collagenase

0.25%

-

Sigma, cat 4188

type IV

TFLLR-NH₂

100 μM

PAR1

Tocris, cat 1464

SFLLRN-OH (for rat)

100 μM

PAR1

Bachem, cat H8365

SLIGRL-NH₂

100 μM

PAR2

Tocris, cat 1468

AYPGKF-NH₂

200 μM

PAR4

Sigma, cat A3227

Vellani et al. Molecular Pain 2010 6:61   doi:10.1186/1744-8069-6-61