GSE suppresses basal CGRP expression in the TNC. (A) An image (40×) of a section of spinal cord 4 mm from the obex stained with the nuclear dye DAPI is shown. (A') An enlarged image of the area of spinal cord that contains the TNC (yellow box in A). (B) Sections of spinal cord obtained from untreated animals (CON) or treated with GSE for 14 days (GSE) and stained for CGRP are shown in the top panels (200×). The bottom panels represent a merged image of the same sections co-stained with CGRP and DAPI. (C) The top panels show images (400×) of TNC stained with antibodies against CGRP. The bottom panels represent the same sections stained for CGRP and DAPI. (D) The average fold change ± SEM of CGRP staining intensity from control values, which were made equal to one, is reported (n = 3 independent experiments) * P < 0.01 when compared to control. Magnification bar = 50 μm.
Cady et al. Molecular Pain 2010 6:91 doi:10.1186/1744-8069-6-91