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Ablation of rat TRPV1-expressing Adelta/C-fibers with resiniferatoxin: analysis of withdrawal behaviors, recovery of function and molecular correlates

Kendall Mitchell1, Brian D Bates1, Jason M Keller1, Matthew Lopez1, Lindsey Scholl1, Julia Navarro1, Nicholas Madian1, Gal Haspel4, Michael I Nemenov23 and Michael J Iadarola1*

Author Affiliations

1 Neurobiology and Pain Therapeutics Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, USA

2 Department of Anesthesia, Stanford University, Palo Alto, CA, USA

3 Lasmed LLC, 137 Irene Ct., Mountain View, CA, USA

4 Laboratory of Neural Control, Section on Developmental Neurobiology, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, USA

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Molecular Pain 2010, 6:94  doi:10.1186/1744-8069-6-94

Published: 17 December 2010

Additional files

Additional file 1:

Aδ laser stimulation of rat hind paw captured with high-speed videography. The rat was recorded at a rate of 500 frames per sec (fps) to capture the first-observable movement after laser stimulation (6.08 W/mm2). The laser stimulus (100 ms, 31 frames) can be easily observed since infrared light saturates the CCD sensor, appearing as black pixels in the video. The rat was at rest and motionless immediately before stimulation. Post-stimulation, ostensible movement could be detected in the left forearm and the right shoulder by 104 ms. Clear movement of the contralateral paw could be detected by 4 frames (113 ms). Complete limb withdrawal and paw licking occur several frames later. The total length of the recording is 1 sec. Withdrawal of the stimulated limb appears rapid and very brisk when viewed in real-time. [Note: Due to video compression, this reformatted video file plays at ~310 fps.]

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Additional file 2:

Upregulation of galanin following intraplantar injection of RTX (50 ng). Gel image shows ganglionic expression levels of mRNA encoding galanin 5 or 10 days (A and B, respectively) after vehicle or RTX treatment, taken from left and right L4-L5 dorsal root ganglia (n = 4 rats, ipsilateral RTX expression is denoted by an asterisk). Graph in (C) shows that galanin transcript levels (after GPDH normalization) were significantly altered by intraplantar RTX injection. Graph is presented as mean ± SEM. (N = 4/group). ***P < 0.001 as determined by one-way ANOVA followed by a Bonferroni correction. ATF3 transcript levels were also increased on day 5 whereas MCP-1 levels were not (data not shown).

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