Sodium channel immunolabeling in rat cerebellum and DRG. Isoform-specific antibodies generated against sodium channels Nav1.1, Nav1.2, Nav1.6 and Nav1.7 were reacted with sections of adult rat cerebellum and DRG. Purkinje cell bodies and apical dendrites exhibit robust Nav1.1 immunolabeling, while limited Nav1.1 immunoreactivity is displayed in DRG neurons (inset). Parallel fibers of cerebellar granule cells exhibit substantial Nav1.2 labeling; Nav1.2 is not detectable in DRG neurons (inset). Nav1.6 is robustly expressed in Purkinje cell bodies and dendrites and is also localized within parallel fibers of cerebellar granule cells; Nav1.6 immunolabeling is exhibited by most neurons within DRG (inset). Within cerebellum, Nav1.7 immunostaining is not detectable, but Nav1.7 immunoreactivity is exhibited by many DRG neurons. The labeling patterns obtained with the isoform-specific sodium channel antibodies Nav1.1, Nav1.2, Nav1.6 and Nav1.7 utilized in these studies is consistent with previous descriptions of their localization within CNS and PNS tissue [4,29].
Ahn et al. Molecular Pain 2011 7:32 doi:10.1186/1744-8069-7-32