Galanin immunoreactivity in laminae I-III. A confocal image taken from a transverse section of the L3 segment that had been reacted to reveal galanin. The solid line represents the outer limit of the grey matter, and the dashed lines show the borders between laminae I, II and III. There is a dense plexus of immunoreactive axons in lamina I and the outer part of lamina II, with some staining in deeper parts of the dorsal horn. Scattered immunoreactive cell bodies are visible, and three of these are marked with arrows. The insets show the two regions outlined by boxes in the main figure, and have been enlarged to show the immunoreactive cell bodies more clearly. The image is a projection of 6 optical sections at 1 μm z-spacing. Scale bar = 100 μm.
Tiong et al. Molecular Pain 2011 7:36 doi:10.1186/1744-8069-7-36