Figure 3.

Lack of co-localisation of galanin, nNOS and parvalbumin in lamina II. a Confocal image showing part of lamina II that has been scanned to reveal galanin (Gal, red). Two galanin-containing cell bodies are visible (arrowheads). b, c The same field scanned for nNOS (green) and parvalbumin (PV, blue). In each case, immunoreactive cell bodies can be seen. d The merged image shows the lack of co-localisation of the three types of immunoreactivity in the cell bodies. The images were obtained from a single optical section. Scale bar = 20 μm.