Figure 5.

Lack of co-localisation of galanin and NPY. a, b, c show a confocal image from laminae I-IIo scanned for galanin (Gal, red), NPY (green) and the neuronal marker NeuN (blue), respectively. d A merged image. One of the NeuN-positive cell bodies is galanin-immuno-reactive (arrow) and another is NPY-immunoreactive (arrowhead). The image is a projection of 3 optical sections at 1 μm z-separation. Scale bar = 20 μm.