Figure 7.

Staining for galanin, NeuN and Sytox in a section used for stereological analysis. a A single confocal optical section through laminae I and II scanned to reveal galanin (Gal, red). b The same field scanned for NeuN (blue), and the nuclear stain Sytox (green). c A merged image. Neuronal nuclei can be recognised by the presence of both NeuN and Sytox staining, and therefore appear cyan, while non-neuronal nuclei are green. Two of the neurons (arrowheads) are immunoreactive for galanin. This appears in a ring surrounding the nucleus, corresponding to the perikaryal cytoplasm. Scale bar = 20 μm.