Co-localisation of galanin with CGRP and VGAT. a, b, c A confocal image through part of lamina II scanned for galanin (Gal, green), CGRP (red) and VGAT (blue), respectively. d A merged image. The small immunoreactive structures correspond to axonal boutons. Several Gal immunoreactive profiles can be seen and most of these are also labelled with the CGRP antibody (two indicated with arrowheads). These appear yellow in d. Two of the galanin-positive boutons (arrows) are also immunostained for VGAT. The image was obtained from 2 optical sections at 0.3 μm z-separation. Scale bar = 5 μm.
Tiong et al. Molecular Pain 2011 7:36 doi:10.1186/1744-8069-7-36