Quantification of TrkA protein content in glabrous skin. TrkA protein was sampled from glabrous skin of same region used for immunocytochemistry. Two bands were recognized by the monoclonal anti-TrkA antibody, a 110 kDa and 140 kDa form corresponding to the immature, unglycosylated form and mature form respectively. TrkA levels decreased significantly at 1 week post-injury and remained significantly lower at 2 weeks post-injury until 4 weeks, at which point no difference was observed compared to sham-operated controls. OD is normalized against β-actin loading controls. ***p < 0.001, **p < 0.01, *p < 0.05; +SEM.
Peleshok and Ribeiro-da-Silva Molecular Pain 2012 8:1 doi:10.1186/1744-8069-8-1